The evaluation of MALDI-TOF and conventional methods for the identification of Trichosporon species isolated from onychomycosis infection
Comparison of the MALDI-TOF and conventional methods
Keywords:MALDI-TOF, Onychomycosis, Trichosporon species, yeast identification
Objective: Onychomycosis is one of the most common nail diseases and accounts for approximately half of all nail abnormalities. It has been noted that yeast-like microorganisms of the genus Trichosporon, which are the cause of uncommon but medically important infections, have increased in recent years as fungal agents causing onychomycosis. In this study, it was aimed to evaluate the MALDI-TOF MS and conventional methods used for identification of Trichosporon species isolated from nail samples.
Methods: The Cerrahpasa Medical Faculty Mycology Laboratory nail sample records of two years were retrospectively reviewed. The performance of conventional methods (morphological and biochemical identification by API 20C AUX), and MALDI-TOF MS method was compared for identification performance at the species level.
Results: The gender distribution of the patients with Trichosporon isolated samples was found as 67% female and 33% male. The anatomical region involvement was 97% toenail and 3% fingernail. Direct microscopy with KOH was found to be positive in 73% of nail samples. Identification performance at the species level of the MALDI-TOF MS method was found higher than the conventional method. 100% of the 33 non-Candida yeasts were defined as Trichosporon spp. with both methods. Among those, 58% were identified at the species level by conventional method and 85% by MALDI-TOF MS method. Non-Candida yeast distribution of 33 isolates by the API 20C AUX method was 40% T. asahii, 12% T. mucoides, 6% T. inkin and it was 40% T. asahii, 30% T. mucoides, 9% T. inkin, 6% Trichosporon debeurmannianum by the MALDI-TOF MS method.
Conclusion: The MALDI-TOF MS method was found to be superior to the conventional method in Trichosporon species identification.
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