The effect of gelatine, pure olive oil and hyaluronic acid on postoperative intraabdominal adhesions: Postoperative intraabdominal adhesions

Yavuz Pirhan; Volkan  Taşova; Fatma Zeynep Özen; Mustafa  Ermiş

doi:10.5281/zenodo.13381661

Authors

Yavuz Pirhan Amasya University, Sabuncuoğlu Şerefeddin Research and Training Hospital, Department of General Surgery, Amasya, Turkey https://orcid.org/0000-0002-8234-6739
Volkan Taşova Amasya University, Sabuncuoğlu Şerefeddin Research and Training Hospital, Department of General Surgery, Amasya, Turkey https://orcid.org/0000-0003-3368-4421
Fatma Zeynep Özen Amasya University, Sabuncuoğlu Şerefeddin Research and Training Hospital, Department of Pathology, Amasya, Turkey https://orcid.org/0000-0003-2468-8375
Mustafa Ermiş Erciyes University Experimental Animals Application and Research Center, Kayseri, Turkey https://orcid.org/0000-0003-2267-1238

DOI:

https://doi.org/10.5281/zenodo.13381661

Keywords:

Intraperitoneal adhesion, hyaluronic acid, pure olive oil, gelatin, Wistar albino rat

Abstract

Objective: In patients who have undergone abdominal surgery, serious adhesions are often encountered in cases of repeated abdominal surgery and problems occur in separating the intraabdominal organs. The aim of this experimental study was to investigate the effects of Gelofusine®, Gelofusine plus pure olive oil, and Gelofusine plus hyaluronic acid administered to the abdomen during operations on intraabdominal adhesions that may occur after surgery.

Methods: Forty adult female Wistar albino rats weighing 200-300 g were used in the study. Rats were randomly divided into 4 separate groups with 10 animals in each group. The abdomen was entered after a 2-cm skin and subcutaneous incision along the midline of the abdomen, and then abrasion was applied to the serosa above the cecum and the right lateral peritoneal surface of the abdomen with a 1-cm child’s toothbrush. In the experimental groups, Gelofusine, Gelofusine plus pure olive oil, or Gelofusine plus hyaluronic acid was applied and the abdomen was anatomically closed with 4/0 Prolene threads in two layers. On the 21st day, all animals were euthanized and evaluated both macroscopically and histopathologically. In the control group, after the abdomen was opened and abrasion was applied, no liquid or other substance was administered and the abdomen was closed.

Results: No adhesions were observed macroscopically in one rat in Group 1, in nine rats in Group 2, in seven rats in Group 3, and in four rats in Group 4. Varying degrees of inflammation were found in the rats of all groups. However, fibrosis was not observed in Group 2. When the groups were compared, there was a statistically significant difference between the control group and the group in which only Gelofusine (Group 2) was applied (p<0.05).

Conclusion: Although significant results were expected in the groups in which pure olive oil and hyaluronic acid were used, only Gelofusine, which contains gelatin as an active ingredient and is used in routine human treatment, was found to be statistically and macroscopically significant in preventing intraabdominal adhesions compared to the other groups.

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