Evaluation of the efficacy of aged garlic extract in reducing neuronal damage in rats with spinal cord ischemia-reperfusion injury : Garlic and ischemia-reperfusion injury

Fahri Eryılmaz

doi:10.5281/zenodo.13736800

Authors

Fahri Eryılmaz Yozgat Bozok University Faculty of Medicine, Department of Neurosurgery, Yozgat, Turkey https://orcid.org/0000-0002-7030-9279

DOI:

https://doi.org/10.5281/zenodo.13736800

Keywords:

Aged garlic extract, antioxidant, ischemia-reperfusion injury, neuronal damage, neuroprotection, spinal cord injury

Abstract

Objective: Aged garlic extract (AGE) is known to improve human well-being via its anti-inflammatory/antioxidant and neuroprotective properties. The purpose of this study was to evaluate the possible pathological, neurological, biochemical, and ultrastructural benefits of AGE in a rat model of spinal cord ischemia-reperfusion (I-R).

Methods: Thirty Sprague-Dawley rats were randomly divided into three groups: I-R, sham (no I-R), and AGE (I-R+AGE), with 10 rats in each group. Neurologically, the rats were assessed with the help of the Basso, Beattie, and Bresnahan (BBB) scoring system. Spinal cord tissue samples were collected for ultrastructural and neurological evaluations. Oxidative markers (malondialdehyde and nitric oxide), antioxidants (glutathione peroxidase, catalase, and superoxide dismutase), inflammatory cytokines (interleukin-1 and TNF-alpha), and caspase-3 were measured.

Results: The AGE group had higher BBB scores in comparison to the I-R group (p<0.05). The AGE group pathologically demonstrated a decreased level of edema of the spinal cord and ischemia (p<0.05). The ultrastructural findings revealed that the tissue structure was preserved in the AGE group. The levels of oxidative markers in the I-R group were higher than those of the other two groups, while the levels of antioxidant enzymes were higher in the AGE group than in the I-R group and the difference between the groups was significant (p<0.05). The sham group and AGE group differed significantly in terms of levels of antioxidant enzymes (p<0.05). Moreover, the administration of AGE reduced caspase-3 activity and inflammatory cytokines in comparison to the I-R group (p<0.05).

Conclusion: This study shows the significant neuroprotective effects of AGE on the pathological, neurological, biochemical, and ultrastructural variables of a rat model of I-R injury of the spinal cord.

References

Abd El-Mottaleb NA, Mahmoud GS, Negm EA, Abdel Maksoud FM. Garlic antagonizes skeletal muscle ischemia reperfusion injury through regulating inflammation, apoptosis and desmin expression in adult male rats. Int J Physiol Pathophysiol Pharmacol. 2019;11:126-37.

Albrakati A. Aged garlic extract rescues ethephon-induced kidney damage by modulating oxidative stress, apoptosis, inflammation, and histopathological changes in rats. Environ Sci Pollut Res Int. 2021;28:6818-29.

Coyoy-Salgado A, Segura-Uribe JJ, Guerra-Araiza C, Orozco-Suárez S, Salgado-Ceballos H, Feria-Romero IA, et al. The Importance of Natural Antioxidants in the Treatment of Spinal Cord Injury in Animal Models: An Overview. Oxid Med Cell Longev. 2019;2019:3642491

Tai H, Jiang XL, Lan ZM, Li Y, Kong L, Yao SC, et al. Tanshinone IIA combined with CsA inhibit myocardial cell apoptosis induced by renal ischemia-reperfusion injury in obese rats. BMC Complement Med Ther. 2021;21:100.

Kanamori Y, Via LD, Macone A, Canettieri G, Greco A, Toninello A, et al. Aged garlic extract and its constituent, S-allyl-L-cysteine, induce the apoptosis of neuroblastoma cancer cells due to mitochondrial membrane depolarization. Exp Ther Med. 2020;19:1511-21.

Shang A, Cao SY, Xu XY, Gan RY, Tang GY, Corke H, et al. Bioactive Compounds and Biological Functions of Garlic (Allium sativum L.). Foods. 2019;8:246.

Shao J, Wu Q, Lv SY, Zhou XM, Zhang XS, Wen LL, et al. Allicin attenuates early brain injury after experimental subarachnoid hemorrhage in rats. J Clin Neurosci. 2019;63:202-8.

Dey A, Nandy S, Mukherjee A, Pandey DK. Plant Natural Products as Neuroprotective Nutraceuticals: Preclinical and Clinical Studies and Future Implications. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2020;90:929-43.

Alrumaihi F. Garlic and its Active Compounds: A Novel Strategy to Fight Diseases Through Modulating Biological Activities. Pharmacogn J. 2020;12:1463-74.

Alquisiras-Burgos I, Ortiz-Plata A, Franco-Pérez J, Millán A, Aguilera P. Resveratrol reduces cerebral edema through inhibition of de novo SUR1 expression induced after focal ischemia. Exp Neurol. 2020;330:113353.

Colín-González AL, Santana RA, Silva-Islas CA, Chánez-Cárdenas ME, Santamaría A, Maldonado PD. The antioxidant mechanisms underlying the aged garlic extract- and S-allylcysteine-induced protection. Oxid Med Cell Longev. 2012;2012:907162.

Van Pelt LF. Ketamine and xylazine for surgical anesthesia in rats. J Am Vet Med Assoc. 1977;171:842-4.

Scheff SW, Saucier DA, Cain ME. A statistical method for analyzing rating scale data: the BBB locomotor score. J Neurotrauma. 2002;19:1251-60.

Kaptanoglu E, Palaoglu S, Surucu HS, Hayran M, Beskonakli E. Ultrastructural scoring of graded acute spinal cord injury in the rat. J Neurosurg. 2002;97:49-56.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351-8.

Keshavarzi Z, Shakeri F, Barreto GE, Bibak B, Sathyapalan T, Sahebkar A. Medicinal plants in traumatic brain injury: Neuroprotective mechanisms revisited. Biofactors. 2019;45:517-35.

Bisdas T, Panuccio G, Sugimoto M, Torsello G, Austermann M. Risk factors for spinal cord ischemia after endovascular repair of thoracoabdominal aortic aneurysms. J Vasc Surg. 2015;61:1408-16.

Gao W, Wang W, Liu G, Zhang J, Yang J, Deng Z. Allicin attenuated chronic social defeat stress induced depressive-like behaviors through suppression of NLRP3 inflammasome. Metab Brain Dis. 2019;34:319-29.

Ommati MM, Azarpira N, Khodaei F, Niknahad H, Gozashtegan V, Heidari R. Methylene blue treatment enhances mitochondrial function and locomotor activity in a C57BL/6 mouse model of multiple sclerosis. TIPS;6:29-42.

Cui X, Lin Q, Liang Y. Plant-Derived Antioxidants Protect the Nervous System From Aging by Inhibiting Oxidative Stress. Front Aging Neurosci. 2020;12:209.

Sciarretta S, Forte M, Castoldi F, Frati G, Versaci F, Sadoshima J, et al. Caloric restriction mimetics for the treatment of cardiovascular diseases. Cardiovasc Res. 2021;117:1434-49.

Khadka B, Lee JY, Park DH, Kim KT, Bae JS. The Role of Natural Compounds and their Nanocarriers in the Treatment of CNS Inflammation. Biomolecules. 2020;10:1401.

Iside C, Scafuro M, Nebbioso A, Altucci L. SIRT1 Activation by Natural Phytochemicals: An Overview. Front Pharmacol. 2020;11:1225.

Silva-Islas CA, Chánez-Cárdenas ME, Barrera-Oviedo D, Ortiz-Plata A, Pedraza-Chaverri J, Maldonado PD. Diallyl Trisulfide Protects Rat Brain Tissue against the Damage Induced by Ischemia-Reperfusion through the Nrf2 Pathway. Antioxidants (Basel). 2019;8:410.

Lee J, Jeong MI, Kim HR, Park H, Moon WK, Kim B. Plant Extracts as Possible Agents for Sequela of Cancer Therapies and Cachexia. Antioxidants (Basel). 2020;9:836.